Prussian Blue-Modified Electrodes for Alzheimer’s Detection

[Mindy]

Prussian Blue (PB) was effectively utilized for the modification of carbon screen-printed electrodes (SPE), enabling enhanced electrochemical detection of amyloid-β (Aβ) peptides through flow injection amperometry. The PB-modified electrodes (ΓPB = 1.9-2.5 nmol·cm⁻²) demonstrated excellent redox performance in phosphate buffer (pH 6.0, 0.1 M KCl), showing two well-defined redox couples: PB/Prussian White (PW) and Berlin Green (BG)/PB, with formal potentials of 119 mV and 817 mV, respectively.
The modified SPEs showed remarkable catalytic enhancement (up to 27-fold increase in current) for Aβ peptide variants lacking the Tyr-10 residue, such as Aβ(1-7)-D7H and rat Aβ(1-16). Cyclic voltammetry confirmed the high reversibility and conductivity of the PB film, evidenced by small peak separations (ΔEp as low as 8 mV) and peak current ratios (Ip,a/Ip,c ≈ 1) over a sweep rate range of 2-200 mV·s⁻¹.
The log-log plots of peak current versus sweep rate yielded slopes near 1, characteristic of adsorption-controlled processes, indicating strong and stable anchoring of PB on the electrode surface. This system enables sensitive detection of peptides that lack inherently electroactive residues, suggesting a valuable tool for electrochemical biosensing of modified or truncated peptides, with potential applications in Alzheimer's research.
This study highlights the utility of Prussian Blue in developing high-performance electrochemical sensors for challenging biomolecular targets.

[Aura Lee]

Semax Polska to nowoczesny suplement wspierający koncentrację, pamięć i odporność organizmu. Produkt jest bezpieczny i dostępny w formie kropli lub kapsułek. Semax Polska pomaga rawić funkcje poznawcze i zwiększa efektywność codziennej pracy. https://semaxpolska.com/wiele-koloro...e-wyjasnienie/